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Thin-layer chromatography analysis of reaction mixtures after 24 h of incubation. (a) Lanes 1 to 3, standard sugars, including lactose (lane 1), glucose (lane 2), and galactose (lane 3); lanes 4 and 5, reaction mixtures from aqueous phases with spore-displayed β-Gal (lane 4) and free β-Gal (lane 5). (b) Lane 1, reaction mixture from organic phase with spore-displayed β-Gal; lane 2, reaction mixture from organic phase with free β-Gal.
