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. 2006 Dec 22;73(7):2251–2256. doi: 10.1128/AEM.01489-06

TABLE 1.

Solvent stabilities of spore-displayed β-Gal on different B. subtilis spores and free-form enzymes in various organic solvents after 1 h of incubation at 37°C

Solvent Solvent hydrophobicity (log P value) Relative activities of β-Gal
Displayed on DB104 spores Displayed on WB700 spores Free form
Control 100 100 100
n-Hexane 3.5 100 100 84.3
Ethyl ether 0.8 77.2 97.0 48.2
Toluene 2.5 51.6 78.8 4.2
Ethyl acetate 0.68 9.6 10.6 0.1
Acetonitrile −0.33 0.8 8.3 0.0
Ethanol −0.24 0.0 0.0 0.0