Figure 2.

NF-κB inhibition down-regulated specific gene expression. (A) FACs analysis of ICAM-1 on cells grown in Tc⁺ (dashed line) or Tc- (solid line) media for 3 days. (B) Western blot analysis of extracts were examined for endogenous IκBα, TRAF1, and p53 expression. Lanes 1, 3, and 5 are extracts from cells grown in Tc⁺ media; lanes 2, 4, and 6 are from cells grown in Tc⁻ media. Lanes 1 and 2, day 1; lanes 3 and 4, day 2; and lanes 5 and 6, day 3. (C) c-Myc and LMP-1 expression was unaffected by inhibition of NF-κB. Lanes are as noted in B.