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PARP cleavage occurred before detectable activation of caspase-8 or Bid cleavage. Western blot analysis of extracts for pro-caspase 8, Bid, and PARP. Lanes 1, 3, and 5 are extracts from cells grown in Tc+ media. Lanes 2, 4, and 6 are from cells grown in Tc− media. Lanes 1 and 2, day 1; lanes 3 and 4, day 2; and lanes 5 and 6, day 3.
