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. 2006 Dec 8;189(5):1573–1581. doi: 10.1128/JB.01675-06

FIG. 2.

FIG. 2.

SDS-PAGE of cell extracts from E. coli harboring γ-resorcylate catabolic genes. (A) Lane 1, molecular weight marker (Fermentas International, Inc.); lane 2, cell extract of E. coli BL21 Star (DE3) harboring pGRA (38.1 μg); lane 3, cell extract of E. coli BL21 Star (DE3) harboring pGRC (21.7 μg); lane 4, cell extract of E. coli BL21 Star (DE3) harboring pGRB (39.2 μg). (B) Lane 1, molecular weight marker (Fermentas International, Inc.); lane 2, cell extract of E. coli BL21 Star (DE3) harboring pGRD (16.6 μg); lane 3, insoluble fraction of E. coli BL21 Star (DE3) harboring pGRD; lane 4, cell extract of E. coli BL21 (DE3) harboring pGRD and the chaperone plasmid pG-Tf2 (32.8 μg); lane 5, insoluble fraction of E. coli BL21(DE3) harboring pGRD and the chaperone plasmid pG-Tf2. (C) Lane 1, molecular weight marker (Bio-Rad Laboratories, Inc.); lane 2, cell extract of E. coli BL21 Star (DE3) harboring pGRDA (24.6 μg); lane 3, insoluble fraction of E. coli BL21 Star (DE3) harboring pGRDA.