TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| Rhizobium sp. strain MTP-10005 | γ-Resorcylate decarboxylase-producing bacterium | 38 |
| E. coli BL21 (DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3) | EMD Biosciences |
| E. coli BL21 Star (DE3) | F−ompT hsdSB (rB− mB−) gal dcm rne131 (DE3) | Invitrogen |
| E. coli NovaBlue | Tcr; endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+lacIqZΔM15::Tn10 (Tcr)] | EMD Biosciences |
| Plasmids | ||
| Vector plasmids | ||
| pET3b | Apr; expression vector | EMD Biosciences |
| pET14b | Apr; expression vector | EMD Biosciences |
| pT7Blue T-Vector | Apr; TA cloning vector | EMD Biosciences |
| Plasmids used for cloning of γ-resorcylate degradation genes | ||
| pGWU1 | Apr; pT7Blue T-Vector, a 1.2-kb SalI fragment | This study |
| pGWU2 | Apr; pT7Blue T-Vector, a 0.75-kb PstI fragment | This study |
| pGWU3 | Apr; pT7Blue T-Vector, a 1.0-kb SalI fragment | This study |
| pGWU4 | Apr; pT7Blue T-Vector, a 0.75-kb BamHI fragment | This study |
| pGWD1 | Apr; pT7Blue T-Vector, a 3.0-kb EcoRI fragment | This study |
| pGWD2 | Apr; pT7Blue T-Vector, a 1.5-kb EcoRI fragment | This study |
| pGWD3 | Apr; pT7Blue T-Vector, a 2.0-kb PstI fragment | This study |
| Plasmids used for expression of γ-resorcylate catabolic genes | ||
| pGRA | Apr; pET14b-graA | This study |
| pGRA3 | Apr; pET3b-graA | This study |
| pGRB | Apr; pET14b-graB | This study |
| pGRC | Apr; pET14b-graC | This study |
| pGRD | Apr; pET14b-graD | This study |
| pGRDA | Apr; pET14b-graDA | This study |
| Plasmids used for qRT-PCR | ||
| pTGF | Apr; pT7Blue T-Vector-graF | 38 |
| pTR16 | Apr; pT7Blue T-Vector-16S rRNA | 38 |