TABLE 1.
Straina | Phenotype or genotype | Relevant characteristicsb | Source or reference |
---|---|---|---|
UM44 | Ind− | Weybridge strain; pXO1+ pXO2− | C. Thorne |
UT53 | atxA | Tox− Kmr | 15 |
UT133 | cya::lacZ | Emr Kmr; UM44 transduced with CP51 (55) propagated on RBAF 144 (48) | This work |
UT147 | pag::lacZ | Emr Kmr; UM44 transduced with CP51 (55) propagated on RBAF 140 (48) | This work |
UT148 | lef::lacZ | Emr Kmr; UM44 transduced with CP51 (55) propagated on RBAF 143 (48) | This work |
UT157 | spo0A | Spo− Spcr | 46 |
UT166 | abrB | Spcr | 46 |
UT198 | sigH | Spo− Kmr | This work |
UT199 | sigH pagA::lacZ | Spo− Kmr Emr; UT147 transduced with CP51 (55) propagated on UT198 | This work |
UT200 | sigH lef::lacZ | Spo− Kmr Emr; UT148 transduced with CP51 (55) propagated on UT198 | This work |
UT201 | sigH cya::lacZ | Spo− Kmr Emr; UT133 transduced with CP51 (55) propagated on UT198 | This work |
UT285 | abrB spo0A | Spo− Spcr Kmr; abrB was deleted in UT157 and is replaced by Ω-km2 | This work |
UT290 | spo0A sigH | Spo− Spcr Kmr; UT157 was transduced using CP51 phage (55) propagated on UT198 | This work |
UT291 | abrB sigH | Spo− Spcr,Kmr; UT66 was transduced using CP51 phage (55) propagated on UT198 | This work |
UT301 | sigH plcR::sigH | Spo+ Spcr Kmr; sigH gene introduced in the plcR locus using pUTB1 | This work |
All UT mutants listed are derivatives of Weybridge strain UM44.
Abbreviations: Ind−, indole auxotrophy; Tox−, toxin production deficiency; Emr, erythromycin resistance; Kmr, kanamycin resistance; Spcr, spectinomycin resistance; Spo−, sporulation deficiency.