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(A) Time course of isomerization of aldopentoses (d-lyxose, l-ribose, and d-mannose) to aldoketoses (d-xylulose, l-ribulose, and d-fructose) by CLLI. The reaction mixtures (1 ml) contained 50 mM sodium phosphate buffer (pH 6.5), 10 mM d-lyxose, l-ribose, or d-mannose, 1 mM Mn2+, and 0.5 mg of CLLI. Aliquots were withdrawn after various times of incubation at 60°C. (B) GC-MS chromatograms of isomerization products from the reaction mixtures with d-lyxose and l-ribose as substrates.
