FIG. 6.

lyxA gene of C. laevoribosii enables E. coli BL21(DE3) to grow on d-lyxose or l-ribose as a sole carbon source. (A to D) Cells were streaked on minimal M9 agar plates supplemented with d-glucose, d-lyxose, or l-ribose as the sole carbon source and incubated at 37°C (see Materials and Methods). (E) To obtain growth curves of E. coli BL21(DE3)/pET-CLRI-549 on minimal M9 media with various sugars as carbon sources, inocula (0.14 × 10⁸ cells) were grown on minimal M9 medium containing 0.4% glucose, washed, and transferred into minimal M9 medium containing 0.4% l-ribose, d-lyxose, or d-glucose, and then the absorbance at 600 nm of the cultures was monitored.