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. 2006 Dec 22;189(5):1774–1782. doi: 10.1128/JB.01395-06

FIG. 2.

FIG. 2.

Screen for enhanced Fe(II) oxidation activity in SB1003. After a ∼20-h incubation in assay buffer containing 0.2 mM Fe(II), SW2 oxidizes an average of about sixfold more Fe(II) than SB1003. This difference in Fe(II) oxidation is clearly visualized upon the addition of the colorimetric reagent ferrozine, where wells containing Fe(II) turn purple and wells with no Fe(II) remain clear. Likewise, during the screen, Fe(II)-oxidizing clones like SB1003/9E12 are identified as clear wells upon addition of ferrozine.