TABLE 2.

Oligonucleotides used in this study^a

Oligonucleotide name	Description	Sequence (5′-3′)
9	Forward primer used to amplify pfoxE insert	cgcccaattgctgcagTTATTGCGCCCTTTTTCATC
10	Reverse primer used to amplify pfoxE insert	ggcgactagtAGGCGATCCTCACCATAGG
13	Forward primer used to amplify pfoxY insert	cgcccaattgctgcagTCCACGAGCTGAACCTGAC
14	Reverse primer used to amplify pfoxY insert	ggcgactagtGTTATAGGTGGCGGTTGCTG
17	Forward primer used to amplify pfoxZ insert	cgcccaattgctgcagTCAGACCACCGATTACGACA
18	Reverse primer used to amplify pfoxZ insert	ggcgactagtGGTTTGAGTTTGAGGCAGGA
21	Forward primer used to amplify pfoxEYZ insert	cgcccaattgctgcagAGGAAGTGCTGACCGACATC
18	Reverse primer used to amplify pfoxEYZ insert	ggcgactagtGGTTTGAGTTTGAGGCAGGA
1	Used for RT-PCR	AAGGTGTTCCAGCACCTGAC
2	Used for RT-PCR	GGCATAGGCGATGATGGTAT
3	Used for RT-PCR	CCTTTGACGGCAAGCTTTAT
4	Used for RT-PCR	GTGATCACCTTGACCAGCAG
5	Used for RT-PCR	CGATCAAGGAATGGATCCTG
6	Used for RT-PCR	GGCACACCGATCTGAATCTT
7	Used for RT-PCR	CGAGTTGTGGAGCTTTTACG
8	Used for RT-PCR	CAGGGCGTTGGAGAAGAAC
54	Forward primer used to verify transcription of foxZ from pfoxZ in SB1003	TTTCATCAACTCGCAACTGG
55	Reverse primer used to verify transcription of foxZ from pfoxZ in SB1003	ATAAAGCTTGCCGTCAAAGG
29	Forward primer used to verify transcription of foxY from pfoxY in SB1003	GACCCTGTGCTATGCCATC
51	Reverse primer used to verify transcription of foxY from pfoxY in SB1003	GGCACCTGGTAGTTCGACAG

^aNucleotides in lowercase denote engineered restriction site sequences. Oligonucleotides 9, 13, 17, and 21 have MfeI and PstI restriction sites at the 5′ end. Oligonucleotides 10, 14, and 18 have an SpeI restriction site at the 5′ end.