TABLE 1.

Determination of mutation rate of M. smegmatis carrying pMORF to Km resistance by fluctuation analysis

Fluctuation expt no.	Strain	No. of cultures	Nt valuea	Lea-Coulson m valueb	Mutation ratec
1	mc2155::pMORF	13	1.0 × 109 ± 0.4 × 109	31	2.1 × 10−8
2	mc2155::pMORF	6	1.7 × 109 ± 0.7 × 109	62	2.5 × 10−8
3	ΔrecA::pMORF	5	1.6 × 108 ± 0.4 × 108	76	3.3 × 10−8
4	ΔrecA::pMORF	5	4.3 × 108 ± 0.4 × 108	81	1.3 × 10−8

^aFinal number of cells in the culture.

^bNumber of mutations per culture.

^cProbability of mutation per cell per generation.