FIG. 1.

B. burgdorferi (Bb) rpoS promoter region. The top line indicates the consensus −24/−12 RpoN-binding site (34), which is highlighted in bold in the rpoS promoter region beneath it. The lowercase letter “a” indicates the transcript start site (mRNA) experimentally determined by 5′-RACE analysis in this study. The relevant sequence changes in double-stranded oligonucleotides designed for this study are shown below the consensus region. The boxes highlight base substitutions, whereas the dashes identify base deletions.