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EMSAs demonstrating binding of E. coli RpoN to the B. burgdorferi rpoS promoter region (40-bp oligonucleotide). Lanes 1, 2, and 3 represent increasing amounts (μg) of E. coli RpoN protein (Ec); lane 4 contains a 50-fold excess of unlabeled double-stranded oligonucleotide (rpoS); lane 5 contains a 50-fold excess of sheared salmon sperm DNA (SS); and lane 6 contains 5 μg of bovine serum albumin (BSA) in place of E. coli RpoN.
