TABLE 1.
Swarming-deficient P. aeruginosa Tn5-luxCDABE transposon mutants identified in this study
| Locus of Tn5 insertion | Function | Polar effect possiblea | Hits of individual transposon mutantsb | Colony diam (mm)c after 24 h
|
||
|---|---|---|---|---|---|---|
| Swarmingd | Swimming | Twitching | ||||
| None (strain PAO1) | 34 ± 4 | 23 ± 1 | 21 ± 1 | |||
| PA3817 | Unknown | Y | 4 ± 1 | 11 ± 1 | 18 ± 1 | |
| PA5208 | Unknown | Y | 4 ± 1 | 23 ± 2 | 22 ± 1 | |
| PA5261 (algR) | Transcriptional regulator | N | 5 ± 1 | 21 ± 1 | 3 ± 1 | |
| PA4554 (pilY1) | Type IV pilus biogenesis | Y | 5 | 5 ± 1 | 22 ± 2 | 3 ± 1 |
| PA2965 (fabF1) | Fatty acid metabolism | Y | 2 | 5 ± 1 | 7 ± 1 | 2 ± 1 |
| PA4551 (pilV) | Type IV pilus biogenesis | Y | 2 | 6 ± 1 | 24 ± 1 | 2 ± 1 |
| PA0186 (atsR) | Unknown | N | 2 | 6 ± 1 | 25 ± 2 | 18 ± 1 |
| PA1100 (fliE) | Flagellar biogenesis | Y | 6 ± 1 | 4 ± 1 | 19 ± 1 | |
| PA0944 (purN) | Nucleotide metabolism | Y | 2 | 7 ± 1 | 14 ± 1 | 20 ± 1 |
| PA4556 (pilE) | Type IV pilus biogenesis | Y | 3 | 7 ± 1 | 27 ± 2 | 2 ± 1 |
| PA5117 (typA) | Regulatory protein | N | 7 ± 1 | 24 ± 1 | 21 ± 1 | |
| PA4526 (pilB) | Type IV pilus biogenesis | Y | 4 | 7 ± 1 | 23 ± 1 | 3 ± 1 |
| PA1452 (flhA) | Flagellar biogenesis | Y | 7 ± 1 | 6 ± 1 | 22 ± 1 | |
| PA4547 (pilR) | Type IV pilus biogenesis | N | 8 ± 2 | 22 ± 1 | 3 ± 1 | |
| PA4546 (pilS) | Type IV pilus biogenesis | Y | 3 | 8 ± 1 | 25 ± 1 | 3 ± 1 |
| PA1087 (flgL) | Flagellar biogenesis | Y | 8 ± 1 | 9 ± 1 | 21 ± 1 | |
| PA3476 (rhlI) | Autoinducer synthesis protein | Y | 9 ± 1 | 22 ± 2 | 19 ± 2 | |
| PA2634 | Unknown | N | 2 | 10 ± 1 | 12 ± 1 | 18 ± 2 |
| PA0428 (rhlE) | ATP-dependent RNA helicase | N | 2 | 10 ± 2 | 21 ± 1 | 19 ± 1 |
| PA0413 (pilL) | Type IV pilus biogenesis | Y | 2 | 10 ± 1 | 22 ± 1 | 4 ± 1 |
| PA1084 (flgI) | Flagellar biogenesis | Y | 2 | 10 ± 1 | 4 ± 1 | 18 ± 1 |
| PA5197 (rimK) | RNA modification | N | 10 ± 1 | 13 ± 1 | 19 ± 3 | |
| PA1086 (flgK) | Flagellar biogenesis | Y | 4 | 12 ± 1 | 7 ± 1 | 20 ± 2 |
| PA1081 (flgF) | Flagellar biogenesis | Y | 12 ± 1 | 8 ± 1 | 19 ± 1 | |
| PA3100 (xcpU) | Protein secretion | Y | 12 ± 1 | 24 ± 2 | 20 ± 1 | |
| PA3912 | Unknown | Y | 13 ± 4 | 13 ± 2 | 19 ± 1 | |
| PA0583 (folK) | Cofactor biosynthesis | N | 3 | 13 ± 2 | 24 ± 1 | 21 ± 2 |
| PA1079 (flgD) | Flagellar biogenesis | Y | 13 ± 3 | 5 ± 1 | 21 ± 1 | |
| PA4701 | Unknown | N | 14 ± 2 | 21 ± 1 | 22 ± 1 | |
| PA4234 (uvrA) | DNA modification | N | 2 | 15 ± 3 | 21 ± 1 | 22 ± 1 |
| PA3352 (flgN) | Flagellar biogenesis | Y | 5 | 16 ± 3 | 8 ± 1 | 20 ± 2 |
| PA0005 (lptA) | Fatty acid metabolism | Y | 16 ± 3 | 18 ± 2 | 16 ± 1 | |
| PA4282 | Unknown | N | 5 | 17 ± 4 | 12 ± 1 | 21 ± 1 |
| PA0887 (acsA) | Central intermediary metabolism | N | 17 ± 4 | 11 ± 1 | 19 ± 1 | |
| PA2840 | RNA processing and degradation | N | 18 ± 2 | 23 ± 1 | 21 ± 1 | |
| PA0407 (gshB) | Amino acid metabolism | N | 2 | 20 ± 2 | 21 ± 1 | 20 ± 1 |
a
Possibility of Tn5 insertion affecting expression on downstream genes. Y, yes; N, no.
b
Hits of individual Tn5 insertion mutations mapped to the same gene identified during the screening.
c
The means ± standard deviations were determined from five different experiments.
d
All swarming-deficient mutants exhibited a t test P value of <0.01 compared to the wild type, which was statistically significant.