TABLE 1.
Results of Tn5 mutant screen for C. crescentus xyl and xyl(Tox) mutants
| Phenotypea | Interrupted gene | No. of isolates | Annotation | Proposed function |
|---|---|---|---|---|
| Xyl− Gluc+ | xylX (CC0823) | 2 | Conserved hypothetical protein | Unknown |
| [Xyl(Tox)] | xylA (CC0822) | 2 | Aldehyde dehydrogenase | α-Ketoglutaric semialdehyde dehydrogenase |
| xylB (CC0821) | 1 | Oxidoreductase, short-chain dehydrogenase/reductase family | Xylose dehydrogenase | |
| xylC (CC0820) upstream regionb | 1 | CC0820: “SMP/Cgr family” | Xylonolactonase | |
| xylD (CC0819) | 1 | Dehydratase (IlvD/Edd family) | Xylonate dehydratase | |
| fbp (CC1385) | 1 | Fructose-1,6-bisphosphatase (EC 3.1.3.11) | Gluconeogenesis | |
| Xyl− Gluc+ | ppdK (CC1471) | 4 | Pyruvate phosphate dikinase (EC 2.7.9.1) | Gluconeogenesis |
| maeB (CC2622) | 1 | NADP-dependent malic enzyme (EC 1.1.1.40) | Gluconeogenesis | |
| fbaA (CC3250) | 2 | Fructose-bisphosphate aldolase (EC 4.1.2.13) | Gluconeogenesis | |
| CC3364 | 1 | Homoserine kinase (EC 2.1.7.13) | Unknown | |
| Xyl− Gluc− | eno (CC1724) | 2 | Enolase (EC 4.2.1.11) | Glycolysis and gluconeogenesis |
| pgk (CC3249) | 4 | Phosphoglycerate kinase (EC 2.7.2.3) | Glycolysis and gluconeogenesis | |
| Xyl+ Gluc− | zwf (CC2057) | 1 | Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) | Entner-Doudoroff pathway |
| CC2056 | 1 | 6-Phospho-glucono-lactonase (EC 3.1.1.31) | Entner-Doudoroff pathway | |
| ppc (CC1493) | 2 | Phosphoenolpyruvate carboxylase (EC 4.1.1.31) | Anaplerotic function | |
| CC3065 | 1 | Transcriptional regulator, LacI family | Unknown | |
| Xyl+ Gluc− [Gluc(Tox)] | eda (CC1495) | 1 | 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.2.14) | Entner-Doudoroff pathway |
a
The “Xyl−” phenotype refers to strains that were unable to grow on M2 medium containing 10 mM d-xylose as the sole carbon source. The “Gluc−” phenotype refers to strains that were unable to grow on M2 medium containing 10 mM d-glucose as the sole carbon source. The “Xyl(Tox)” phenotype refers to strains that were sensitive to the presence of d-xylose in the medium, i.e., strains that were able to grow on M2G but that did not form colonies when 10 mM d-xylose was added to M2G.
b
Tn5 insertion was between the CC0821 and CC0820 coding regions.