TABLE 1.

Strains and plasmids

Strain or plasmid	Relevant characteristic(s)	Reference or source
M. xanthus strains
DK1622	Wild type	26
DK5208	Tn5-132::csgA	35
DK7853	asgA476	15
DK10602	DK1622::pTG021	This work
DK10603	ΔactB	17
DK10604	ΔactC	17
DK10605	ΔactA	17
DK10627	Mx8[MS1512] → DK10605a	This work
DK10641	DK1622::pREG1727	This work
DK10642	DK1622 attMx8::pTG027	This work
DK10643	Mx8[DK10642] → DK5208	This work
DK10644	Mx8[DK10642] → DK7853	This work
DK10645	DK10642::pEE106; SalI-NdeI	This work
DK10646	Mx8[DK10642] → DK10605	This work
DK10647	Mx8[DK10642] → DK10603	This work
DK10648	Mx8[DK10642] → DK10604	This work
DK10649	Mx8[DK10642] → MS1512	This work
DK10650	Mx8[DK10642] → fruA::TcΩV	47 and this work
DK10651	Mx8[DK10602] → DK10605	This work
DK10652	Mx8[DK10602] → DK10603	This work
DK10653	Mx8[DK10602] → DK10604	This work
DK10654	Mx8[DK10602] → DK5208	This work
DK10655	Mx8[DK10602] → DK7853	This work
DK10658	DK1622 attMx8::pTG033	Table S2b
DK10659	DK1622 attMx8::pTG037	enh1-1 (Table S2)
DK10660	DK1622 attMx8::pTG038	enh2-1 (Table S2)
DK10661	DK1622 attMx8::pTG039	σ54 5 C→T (Table S2)
DK10662	DK1622 attMx8::pTG040	σ54 6 C→Δ (Table S2)
DK10665	DK1622::pTG042	σ54 1 T→C (Table S2)
DK10666	DK1622::pTG043	σ54 7 T→C (Table S2)
DK10667	DK1622::pTG044	σ54 2 G→T (Table S2)
DK10668	DK1622::pTG045	σ54 3 G→T (Table S2)
DK10669	DK1622::pTG046	σ54 4 C→T (Table S2)
DK10670	DK1622::pTG047	enh1-2 (Table S2)
DK10671	DK1622::pTG048	enh2-2 (Table S2)
DK10672	DK1622::pTG049	enh3 (Table S2)
DK10673	DK1622::pTG050	C box 1 (Table S2)
DK10674	DK1622::pTG051	C box 2 (Table S2)
DK10675	DK1622::pTG052	σ54 8 G→T (Table S2)
MS1512	sdeK (Ω4408)	11
Plasmids
pBluescriptII SK+	3.0 kb; Ampr (bla) lacZ with multiple cloning site for blue-white screening	Stratagene
pEE106	Promoter and part of fruA gene in pBGS18 (59)	8
pJBZ281	7.2 kb; Kanr; polylinker sites upstream of lacZ for translational fusions	M. R. K. Alley
pREG1727	20.7 kb; Ampr Kanr Mx8 attachment site attP; transcriptional terminators followed by multiple cloning sites upstream of promoterless lacZYA genes for transcriptional fusions	R. Gill (10)
pSWU12	pBGS18 with Tn903 Kanr deleted and replaced with pKS-Tetr cassette; MluI/EcoRI	71
pTG021	pJBZ281::pTG108 (sequence bp 496 to 2192); SmaI-SmaI	This work
pTG025	pTG021::pBluescriptII SK+; EcoRI-BamHI	This work
pTG027	pREG1727::pTG025; EcoRI-XhoI	This work
pTG033	pBluescript/pREG1727::PCR fragment with act promoter region from upstream XhoI to downstream BamHI; BamHI-XhoI	This work
pTG108	Deletion of PstI fragment from pLAG66 (13); PstI-PstI	This work

^aCan be read as “a stock of Mx8 grown on MS1512 was used to transduce DK10605.”

^bTable S2 can be found in the supplemental material.