TABLE 2.
Primers employed in the studya
| Primer name | Primer sequence |
|---|---|
| pilG5′XhoI | 5′-ACGACTCGAGATGGCTAAAAACGGAGGAT-3′ |
| pilG3′SacII | 5′-TAGACCGCGGTCAGGCGACCACGTTGCC-3′ |
| pilG3′PsyI | 5′-CGGACAATGTCTCCGAAAATC-3′ |
| A8 | 5′-GCGTCTGAACAAACAGTGATGGATATGTCCG-3′ |
| A9 | 5′-CCGTCTGAACAAACAGTGATGGATATGTCCG-3′ |
| A10 | 5′-GCGCCGTCTGAACAAACAGTGATGGATATGTCCG-3′ |
| A11 | 5′-TGCCGTCTGAACAAACAGTGATGGATATGTCCG-3′ |
| A12 | 5′-ATGCCGTCTGAACAAACAGTGATGGATATGTCCG-3′ |
| B8 | 5′-TTCAGACGCAGGTCAGTGCGGGAAGCTC-3′ |
| B9 | 5′-TTCAGACGGAGGTCAGTGCGGGAAGCTC-3′ |
| B10 | 5′-TTCAGACGGCGCGGTCAGTGCGGGAAGCTC-3′ |
| B11 | 5′-TTCAGACGGCAGGTCAGTGCGGGAAGCTC-3′ |
| B12 | 5′-TTCAGACGGCATGGTCAGTGCGGGAAGCTC-3′ |
| S1 | 5′-TTTTGGTCGTAGAGCACACG-3′ |
| S2 | 5′-AAACATGCAGGAATTGACGA-3′ |
| S3 | 5′-TCGGTTTGGTATTCGTGATG-3′ |
| 2488 | 5′-GTAAAACGACGGCCAGT-3′ |
| 2487 | 5′-AGCGGATAACAATTTCACACAGGA-3′ |
| Single F | 5′-TATGCCGTCTGAAA-3′ |
| Single R | 5′-ATTTCAGACGGCAT-3′ |
| Inverted F | 5′-TATGCCGTCTGAAAGCCTTTCAGACGGCAT-3′ |
| Inverted R | 5′-AATGCCGTCTGAAAGGCTTTCAGACGGCAT-3′ |
| Direct F | 5′-TATGCCGTCTGAAAGCCTATGCCGTCTGAA-3′ |
| Direct R | 5′-ATTCAGACGGCATAGGCTTTCAGACGGCAT-3′ |
a
Restriction sites are underlined, and DUS elements are in bold letters.