FIG. 2.

Temporal gene expression in cells shifted from Bvg⁻-phase conditions to Bvg⁺-phase conditions in RB50 (A), RBL (B), and RBH (C). lacZ fusion strains were grown overnight in SS broth containing 25 mM MgSO₄ and then washed and resuspended in SS broth without added MgSO₄ at time zero, and samples were taken every 1.5 h postshift. Relative expression of frlAB (blue lines), bipA (yellow lines), fhaB (orange lines), and ptxA (red lines) was calculated by setting the maximum β-galactosidase activity of each gene in RB50 (wild type [WT]) to 100%. The light blue background indicates Bvg⁻-phase expression, the yellow background indicates the Bvgⁱ phase, and the light red background indicates the Bvg⁺ phase.