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σB activity in a representative mutant. B. subtilis strains BSJ43 (rsbP::spc SPβ ctc::lacZ) (triangles) and BAR12 [rsbP::spc rsbR(136EK) SPβ ctc::lacZ)] (squares) growing logarithmically in LB medium were sampled at the indicated times and analyzed for σB-dependent β-galactosidase levels (Miller units) as described in Materials and Methods.
