TABLE 4.
σB activity in merodiploid rsbR and rsbS strains during growth
| rsbR/rsbS allelea | β-Galactosidase (ctc::lacZ) activity (Miller units) for indicated duplicated elementb
|
|
|---|---|---|
| PA rsbR rsbS rsbT rsbU | PA rsbR/PA rsbR rsbS | |
| Wild type | 1.2 ± 0.3 | 0.64 ± 0.45/0.76 ± 0.38c |
| rsbR(136EK) | 19.2 ± 0.6 | 1.2 ± 0.02 |
| rsbR(225CY) | 28.5 ± 2.2 | 1.0 ± 0.46 |
| rsbS(22ER) | 42.5 ± 7.2 | 0.67 ± 0.07 |
| rsbS(23LF) | 3.6 ± 0.9 | 0.57 ± 0.45 |
| rsbS(66GR) | 6.6 ± 1.0 | 0.38 ± 0.25 |
| rsbS(76GR) | 3.1 ± 1.1 | 0.40 ± 0.20 |
| rsbS(83GD) | 3.1 ± 0.4 | 0.53 ± 0.33 |
| rsbS(86PL) | 7.3 ± 2.9 | 0.62 ± 0.21 |
a
Each strain contains a wild-type sigB operon plus the indicated rsbR or rsbS allele in a transcription unit containing rsbR rsbS rsbT rsbU or rsbR (for rsbR mutations), rsbR rsbS rsbT rsbU or rsbR rsbS or rsbR rsbS (for rsbS mutations).
b
All strains are RsbP−. The indicated allele is present in the diploid element outside of the sigB operon.
c
Values represent two copies of wild-type rsbR/rsbR rsbS.