FIG. 5.

Colony variant distribution in S. pneumoniae biofilms is affected by treatment with tetracycline. Biofilms were grown in tube reactors for 1 day before treatment with tetracycline (10 μg/ml) for 6 h (A) or for 6 days before treatment with tetracycline (10 μg/ml) for 12 h (B). Inset: distribution of colony variants in 6-day-old S. pneumoniae WT biofilm grown in flow cells before and after treatment with tetracycline. Distribution of colony variants was assessed by total CFU, colony size, and mucoidy on blood agar. Experiments were performed in triplicate.