TABLE 1.
Bacillus subtilis strains and plasmids used in this study
| Strain or plasmid | Relevant phenotype or descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| JH642 | trpC2 pheA1 | J. A. Hoch |
| ORB3621 | trpC2 pheA1 rpoA(Y263C) | 15 |
| ORB3834 | trpC2 pheA1 spx::neo | 14 |
| ORB4888 | trpC2 pheA1 amyE::PyjbC-spx-bgaB cat | This study |
| ORB4889 | trpC2 pheA1 amyE::Pspx-bgaB cat | This study |
| ORB4980 | trpC2 pheA1 amyE::Pspx?−40-bgaB cat | This study |
| ORB4981 | trpC2 pheA1 amyE::PspxΔ−60-bgaB cat | This study |
| ORB4982 | trpC2 pheA1 amyE::PspxΔ−100-bgaB cat | This study |
| ORB5058 | trpC2 pheA1 amyE::PspxΔ+50(wt)-bgaB cat | This study |
| ORB5077 | trpC2 pheA1 amyE::PspxΔ+5-bgaB cat | This study |
| ORB5078 | trpC2 pheA1 amyE::PspxΔ+15-bgaB cat | This study |
| ORB5079 | trpC2 pheA1 amyE::PspxΔ+30-bgaB cat | This study |
| ORB5107 | trpC2 pheA1 amyE::PspxΔ+50(wild type)-bgaB cat spx::neo | This study |
| ORB5115 | trpC2 pheA1 amyE::PspxΔ+40-bgaB cat | This study |
| ORB6030 | trpC2 pheA1 amyE::Pspx(T−26A)-bgaB cat | This study |
| ORB6031 | trpC2 pheA1 amyE::Pspx(T−20G)-bgaB cat | This study |
| ORB6032 | trpC2 pheA1 amyE::Pspx(T−19G)-bgaB cat | This study |
| ORB6033 | trpC2 pheA1 amyE::Pspx(A−14T)-bgaB cat | This study |
| ORB6034 | trpC2 pheA1 amyE::Pspx(A+3G)-bgaB cat | This study |
| ORB6035 | trpC2 pheA1 amyE::Pspx(T+7C)-bgaB cat | This study |
| ORB6036 | trpC2 pheA1 amyE::Pspx(T+24C)-bgaB cat | This study |
| Plasmids | ||
| pSN16 | yjbC-spx-lacZ fusion plasmid | 19 |
| pML11 | yjbC-spx transcriptional fusion in pUC19 | This study |
| pML18 | yjbC-spx transcriptional fusion in pDL | This study |
| pML19 | spx transcriptional fusion in pDL | This study |
| pML20 | spx promoter fragment (−40) in pDL | This study |
| pML21 | spx promoter fragment (−60) in pDL | This study |
| pML22 | spx promoter fragment (−100) in pDL | This study |
| pML23 | yjbC promoter fragment in pDL | This study |
| pML25 | spx promoter fragment (wild type +50) in pUC19 | This study |
| pML26 | spx promoter fragment (wild type +50) in pDL | This study |
| pML27 | spx promoter fragment (+5) in pUC19 | This study |
| pML28 | spx promoter fragment (+15) in pUC19 | This study |
| pML29 | spx promoter fragment (+30) in pUC19 | This study |
| pML30 | spx promoter fragment (+5) in pDL | This study |
| pML31 | spx promoter fragment (+15) in pDL | This study |
| pML32 | spx promoter fragment (+30) in pDL | This study |
| pML33 | spx promoter fragment (+40) in pUC19 | This study |
| pML34 | spx promoter fragment (+40) in pDL | This study |
| pML35 | Random mutagenesis of spx promoter in pUC19 | This study |
| pML36 | Random mutagenesis of spx promoter in pDL | This study |
| pML42 | spx(T−26A) in pDL | This study |
| pML43 | spx(T−20G) in pDL | This study |
| pML44 | spx(T−19G) in pDL | This study |
| pML45 | spx(A3G) in pDL | This study |
| pML46 | spx(A−14T) in pDL | This study |
| pML47 | spx(T7C) in pDL | This study |
| pML48 | spx(T24C) in pDL | This study |
a
wt, wild type.