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Decay of the hasABC transcript expressed from the Psag promoter in stationary-phase cells. (A) Diagram showing pJRS1293. The fusion consists of the entire hasABC transcript (postitions +1 to 3837; gray) fused to the Psag promoter region (black). The lollipop symbols represent predicted Rho-independent transcriptional terminators. RNase protection assays (B) and the decay curve calculations (C) were performed as described for Fig. 1.
