FIG. 5.

Stationary-phase expression of sagA mRNA from different promoters. (A) Diagram of the Phas-sagA and Psag-sagA constructs. The Phas-sagA fusion consists of the entire sagA transcript (+1 to +602; thick black line) fused to the Phas promoter (gray line) by overlap-extension PCR. The loop downstream of sagA represents a transcriptional pause site at which most of the mRNA produced from Psag is terminated in vivo. (B) RPAs were performed on 100 ng RNA. The arrow to the right indicates the expected size of the protected RNA probe.