TABLE 3.
Growth enhancement of Bacillus anthracis by addition of petrobactinBa to 0.5 mM 2, 2′-dipyridyl medium using disc diffusion assaysa
| Strain description | Growth zone (mm) per reagent (n = 5 discs)
|
||
|---|---|---|---|
| H2O | 2 μg petrobactinBa | 10 μg petrobactinBa | |
| Wild type (spores) | No growth | 26.6 ± 2.1 | 32.8 ± 1.9 |
| Δasb::kmr (spores) | No growth | 17.0 ± 1.7 | 25.2 ± 0.5 |
| Wild type (vegetative) | Lawn | Lawna | Lawna |
| Δasb::kmr (vegetative) | No growth | 18.2 ± 0.8 | 24.4 ± 0.9 |
a
Student's t test comparing results for wild-type and Δasb::kmr spores, P < 0 0.01; Student's t test comparing results for Δasb::kmr spores and vegetative cells, P = 0.2.
b
Slightly heavier growth along edges of discs.