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. 2006 Dec 22;189(5):2063–2068. doi: 10.1128/JB.01239-06

FIG. 2.

FIG. 2.

BBA74 does not have surface-exposed epitopes. B. burgdorferi cells were incubated with proteinase K with or without permeabilization. Total lysates were separated on 10 to 20% SDS-PAGE gels and blotted to PVDF membranes. The membranes were probed with polyclonal rat sera against BBA74 or OppA IV or rabbit sera against P66 or FlaB. Lanes 1, 4, 7, and 10, intact cells without proteinase K treatment; lanes 2, 5, 8, and 11, intact cells with proteinase K treatment; lanes 3, 6, 9, and 12, permeabilized cells with proteinase K treatment. Arrowheads indicate native and digested proteins. The migration positions of molecular mass markers are indicated by numbers in the right margin.