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Cellular localization of BBA74. OMV and PC fractions were prepared and treated as described in Materials and Methods. Protein fractions were separated by 12.5% SDS-PAGE, and the indicated proteins were visualized by immunoblotting. (A) Separation into OMV and PC fractions; (B) 1 M NaCl treatment; (C) TX-114 phase partitioning. P, pellet; S, soluble; A, aqueous; D, detergent.
