TABLE 5.
RT-PCR analyses with primers prepared from degradative genes on pCAR3
| Growth substrate | Result obtained with degradative genea:
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| carAaI | carAcI | carAaII | carAcII | carDFE | andA | catBCAD | catIJF | ligAB | ophA | dbfA1A2 | |
| Anthranilate | ND | ND | ND | ND | - | + | + | + | + | + | + |
| Benzoate | ND | ND | ND | ND | - | + | + | + | + | + | + |
| Carbazole | ++ | ++ | ++ | ++ | + | + | + | + | + | + | + |
| Catechol | ND | ND | ND | ND | + | + | + | + | + | + | + |
| Phthalate | ND | ND | ND | ND | ND | + | ND | ND | + | + | ND |
| Protocatechuate | ND | ND | ND | ND | ND | + | ND | ND | + | + | ND |
| Succinate | + | + | + | + | - | + | - | - | + | + | + |
a
ND, not determined. “++” indicates that the amount of mRNA for each gene and condition was more abundant than those determined to be “+” as confirmed by quantitative RT-PCR. A “-” indicates that no amplicons were detected. The results for carAaI, carAcI, carAaII, and carAcII are from Urata et al. (64).