FIG. 3.

Loss of YdcQ downregulates envelope stress responses. (A) LacZ activity of various reporter fusions (shown in the x axis) controlled by envelope stress responses in a wild-type (▪) and a ydcQ::kan (░⃞) strain. The degP′-lacZ⁺ fusion is controlled by both σ^E and Cpx, rpoHP3′-lacZ⁺ by σ^E, cpxP′-lacZ⁺ by Cpx, and spy′-lacZ⁺ by Cpx and Bae. Relative LacZ activities are shown where 100% corresponds to the LacZ activity of the wild-type strain carrying each fusion. Shown is an experiment representative of at least three independent experiments. (B) Western blotting of whole-cell samples showing reduced levels of DegP and Spy in a ydcQ::kan mutant (NR905) with respect to the wild-type (wt) MC4100 and ydcR::kan mutant (NR906) strains.