TABLE 2.
Representative phenotypic classes of rpoE suppressors
Strain genotypea | Growthb |
|||||
---|---|---|---|---|---|---|
SDS/EDTA | 22°C | 37 oC | 42 oC | Glucose | Glycerol | |
rpoE+ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ |
rpoE::cam sup9 | + | + | +++ | + | ++ | ++ |
rpoE::cam sup1 | + | +++ | ++++ | +++ | − | − |
rpoE::cam sup5 | − | + | +++ | ++ | ++ | ++ |
rpoE::cam sup21 | ++ (M) | + (M) | ++++ (M) | + | ++ (M) | + |
rpoE+ refers to NR670. Shown are data derived from a representative suppressor supn for each of the four suppressor classes.
Growth of suppressor strains is reported relative to that of an rpoE+ strain, which was arbitrarily given a “++++” value. No growth is reported as “−”. “(M)” refers to a mucoid growth phenotype. SDS/EDTA = LB agar + 0.5% SDS 0.5 mM EDTA; 22°C, 37°C, and 42°C refer to LB agar incubated at the indicated temperatures; glucose refers to glucose M63 minimal agar; glycerol refers to glycerol M63 minimal agar.