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. 2006 Dec 8;189(5):1589–1596. doi: 10.1128/JB.01151-06

FIG. 3.

FIG. 3.

Ectopic expression of a pduL minimal clone corrects the growth defect of a pta mutant on acetate minimal medium. Data for S. enterica harboring expression vector pLAC22 without an insert (control), a pta mutant carrying pLAC22 without an insert (Pta−/no insert), and a pta mutant harboring a pduL minimal clone in pLAC22 (Pta−/pduL) are shown. The relevant strains are BE287, BE529, and BE530 (Table 1). Controls showed that pLAC22 with or without the pduL insert did not observably affect the growth of the wild-type strain on acetate minimal medium (data not shown). In addition, the growth defect of the pta mutant was fully corrected by a pta minimal clone, showing that the effects of this mutation were not due to polarity (data not shown). The growth medium was NCE supplemented with 0.2% Na acetate and 0.2 mM IPTG. Each growth study was performed twice in quadruplicate. Representative curves are shown. Log-phase doubling times with standard deviations are given in the text. OD600, optical density at 600 nm.