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Propionyl-CoA is produced by the PduL reaction. (A) HPLC-ESI-MS of propionyl-CoA standard. (B) HPLC-ESI-MS of a PduL assay after 10 min of incubation at 30°C. The assay mixture initially contained 10 μg cell extract from BE527 (Pta− PduL+), 0.4 mM HS-CoA, 1 mM propionyl-PO42−, and buffer.
