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Germination of wild-type and TS spoVA spores with dodecylamine. Spores of strains PS832 (wild-type) (○ and ▵) and PS3642 (TS spoVA) (• and ▴) were prepared at 30°C. These spores were germinated with 50 μM dodecylamine in 10 mM Tris-HCl (pH 7.5) at either 30°C (○ and •) or 55°C (▵ and ▴), and DPA release was measured as described in Materials and Methods.
