FIG. 2.

Symptoms induced in pepper leaves (Capsicum annuum cv. ECW-10R) by the X. campestris pv. campestris strains. Approximately 5 μl bacterial culture suspended in 10 mM sodium phosphate buffer was infiltrated into the leaf mesophyll tissue with a blunt-end plastic syringe. Pictures of the pepper leaf were taken at 8, 16, and 24 h after infiltration. Three replications were done in each experiment, and each experiment was repeated three times. The results presented are from a representative experiment, and similar results were obtained in all other independent experiments. The cell concentrations used for inoculation and the observation time (postinoculation) are indicated. (A) hpaR mutant NK2827; (B) complemented strain CNK22827; (C), wild-type strain 8004; (D) type III-deficient strain (hrcV mutant; negative control).