. 2006 Dec 8;189(5):2055–2062. doi: 10.1128/JB.01331-06

TABLE 1.

Bacterial strains and plasmids used in this work

Strain or plasmid	Relevant characteristics^a	Reference or source
E. coli JM109	recA1 endA1 gyrA96 thi supE44 relA1 Δ(lac-proAB)/F′ [traD36 lacI^qlacZ ΔM15]	67
X. campestris pv. campestris strains
8004	Wild type; Rif^r
8004*	8004 derivative containing an E45K conversion in HrpG; Rif^r	15
8004* ΔhrpX	As 8004* but hrpX deleted; Rif^r Kan^r	27
NK2827	As 8004 but hpaR::pK18mob; Rif^r Kan^r	27
NK2827*	As 8004* but hpaR::pK18mob; Rif^r Kan^r	This work
CNK2827	NK2827 harboring pXC2827; Rif^r Kan^r Tc^r	This work
8004G	8004 ΔhrpG; Rif^r Kan^r	This work
8004X	8004 ΔhrpX; Rif^r Kan^r	Laboratory collection
8004pG28	8004 harboring pGUS2827; Rif^r Kan^r Tc^r	Laboratory collection
8004GpG28	8004G harboring pGUS2827; Rif^r Kan^r Tc^r	This work
8004XpG28	8004X harboring pGUS2827; Rif^r Kan^r Tc^r	This work
8004RpG28	NK2827 harboring pGUS2827; Rif^r Kan^r Tc^r	This work
161E12	As 8004 but XC_2826::Tn5gusA5; Rif^r Kan^r Spc^r Gm^r	This work
NK2828	As 8004 but XC_2828::pK18mob; Rif^r Kan^r	Laboratory collection
Plasmids
pLAFR6	Broad-host-range cloning vector; Tc^r	23
pRK2073	Helper plasmid; Tra⁺ Mob⁺ ColE1 Spc^r	30
pK18mob	Suicide plasmid in X. campestris pv. campestris; Kan^r	45
pT18mob	Tetracycline-resistant derivative of pK18mob; Tc^r	Laboratory collection
pK2827	pK18mob containing a 267-bp internal fragment of the hpaR gene; Kan^r	This work
pT2827	pT18mob containing a 267-bp internal fragment of the hpaR gene; Kan^r	This work
pXC2827	pLAFR6 containing a 758-bp fragment including the hpaR gene; Tc^r	This work
pGUS2827	pLAFR6 containing a hpaR promoter-gusA fusion fragment; Tc^r	This work
pGUShrpA	pLAFR6 containing a hrpA operon promoter-gusA fusion fragment; Tc^r	This work
pGUShrpB	pLAFR6 containing a hrpB operon promoter-gusA fusion fragment; Tc^r	Laboratory collection
pGUShrpC	pLAFR6 containing a hrpC operon promoter-gusA fusion fragment; Tc^r	Laboratory collection
pGUShrpD	pLAFR6 containing a hrpD operon promoter-gusA fusion fragment; Tc^r	Laboratory collection
pGUShrpE	pLAFR6 containing a hrpE operon promoter-gusA fusion fragment; Tc^r	Laboratory collection
pGUShrpF	pLAFR6 containing a hrpF operon promoter-gusA fusion fragment; Tc^r	Laboratory collection
pGUShrpG	pLAFR6 containing a hrpG promoter-gusA fusion fragment; Tc^r	Laboratory collection
pGUShrpX	pLAFR6 containing a hrpX promoter-gusA fusion fragment; Tc^r	Laboratory collection

Rif^r, Kan^r, Spc^r, Gm^r, and Tc^r, rifampin, kanamycin, spectinomycin, gentamicin, and tetracycline resistant, respectively.