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Electrophoretic mobility shift assay of probe p97 with the recombinant D1:1 fragments B1, B2, and B3. The sample in lane 1 contained the assay mix without protein. The samples in lanes 2 and 3 included 450 ng of fragment B1. The protein content in lanes 4 and 5 was 740 ng of fragment B2. In lanes 6 and 7, 580 ng of fragment B3 was applied. In lanes 3, 5, and 7, a 70× excess of unlabeled probe p97 was added.
