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Electrophoretic mobility shift assay of probe p97 with recombinant polypeptides B1 to B5 and the pD1:1 protein. The sample in lane 1 contained the assay mix without protein. The sample in lane 2 contained 450 ng of B1 protein. The sample in lane 3 contained 740 ng of B2 protein. In lane 4, 580 ng of recombinant B3 protein was added. Lane 5 contains 1.155 μg of B4. Lane 6 contains 2.535 μg of fragment B5. In lane 7, 168 ng of pD1:1 was added.
