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Gel retardation assay of probe p518 with recombinant proteins B3 and B5. Lane 1 displays the assay mix without protein. Lanes 2 to 4 contain 290 ng B3, and lanes 5 to 7 contain 845 ng of fragment B5. In lanes 3 and 6, a 16× excess of unlabeled probe was added. Lanes 4 and 7 contain a 66× excess of unlabeled p518.
