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. 2006 Dec 22;189(5):2119–2124. doi: 10.1128/JB.01362-06

FIG. 3.

FIG. 3.

The Tsr19-regulated invertible promoter does not affect capsular polysaccharide expression. A. Western immunoblot analysis of whole-cell lysates of the wild type, Δtsr19 M1, and Δtsr19 M3 probed with antisera to each of the eight capsular polysaccharides. B. Analysis of the transcription of mpi and BF2782 by the Tsr19-regulated invertible promoter, using xylE transcriptional fusion clones. pLEC55 detects transcription into mpi when the invertible promoter is oriented toward mpi, and pMCL25 and pMCL26 detect transcription into BF2782 when the invertible promoter is cloned in either the OFF or ON orientation, respectively. The resulting milliunits of XylE activity per milligram protein are reported for each of these constructs when in trans in B. fragilis 9343.