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Casein binding proteins on the P. aeruginosa outer membrane. Outer membrane fractions were isolated from strain PAK grown under type III inducing conditions (lane +) or noninducing conditions (lane −), and Triton X-100-solubilized proteins were subjected to affinity purification using casein-agarose. Specific bound proteins were subjected to 12% SDS—PAGE, followed by silver staining. KD, kilodaltons.
