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Constitutive ExoS secretion by a popN mutant. ExoS secretion by wild-type strain PAK and isogenic popN and pcrV mutant strains, as well as mutants harboring either vector plasmid pUCP19 or vector plasmid pUCP19 containing the corresponding wild-type genes, was examined. Bacterial cells were grown in L broth in the presence or absence of 5 mM EGTA, and secreted ExoS in the culture supernatants was detected by Western blotting using anti-ExoS antibody.
