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. 2007 Jan 19;189(7):2599–2609. doi: 10.1128/JB.01680-06

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Copyright © 2007, American Society for Microbiology

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FIG. 6. — Detection of PopN-Flag and pilin on the bacterial cell surface by whole-cell ELISA. Microplates were coated with 10⁸ bacterial cells grown under type III inducing conditions (+EGTA) or noninducing conditions (−EGTA). PopN-Flag and pili were detected with anti-Flag monoclonal antibody and rabbit anti-PilA polyclonal antibody, respectively. PAK, wild-type P. aeruginosa strain; popN⁻, popN mutant of PAK; pilA⁻, pilA mutant of PAK; pUCP19, vector control; pPppB-Flag, fusion construct with Flag tag at the C terminus of cytoplasmic protein PppB; pPopN-Flag, popN::flag fusion construct. OD₄₉₂, optical density at 492 nm.