TABLE 2.
Interaction study using BacterioMatch two-hybrid system
| pBT plasmid | Interaction witha:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Vector | pTRG-Gal11p | pTRG-popN | pTRG-pcr1 | pTRG-pcr2 | pTRG-pcr3 | pTRG-pcr4 | pTRG-pcrD | pTRG-pcrR | pTRG-pcr2 + pscB | |
| Vector | − | − | − | − | − | − | − | − | − | − |
| pBT-LGF2 | − | +++b | NT | NT | NT | NT | NT | NT | NT | NT |
| pBT-popN | − | NT | − | ++ | − | − | − | − | − | ++ |
| pBT-pcr1 | − | NT | ++ | − | − | − | + | − | − | NT |
| pBT-pcr2 | − | NT | − | − | − | − | − | − | − | NT |
| pBT-pcr3 | − | NT | − | − | − | − | +++ | − | − | NT |
| pBT-pcr4 | − | NT | − | + | − | ++ | − | − | − | NT |
| pBT-pcrD | − | NT | − | − | − | − | − | − | − | NT |
| pBT-pcrR | − | NT | − | − | − | − | − | − | − | NT |
a
−, no interaction (β-galactosidase activity, <180); +, positive interaction with β-galactosidase activity of 250 to 350; ++, positive interaction with β-galactosidase activity of 350 to 450; +++, positive interaction with β-galactosidase activity of >450; NT, not tested.
b
Positive control provided with the kit.