FIG. 3.

Analysis of mRNA expression from the dpkA and ORF3592 promoters in the wild-type and a mutant background with a nonpolar insertion in ORF3592. (A) Promoter structure of the dpkA and ORF3592 genes. The transcription start points were determined by primer extension as detailed in Materials and Methods. The +1 and the potential −10 and −35 hexamers of each promoter are shown. (B and C) Expression of dpkA and ORF3592 promoters in the wild type (lanes 1 to 3) and the mutant deficient in the synthesis of PP3592 (lanes 4 to 6). Lanes 1 and 4, cells grown on M9 minimal medium; lanes 2 and 5, cells grown on M9 minimal medium with 5 mM l-lysine; lanes 3 and 6, cells grown on M9 minimal medium with 5 mM d-lysine.