TABLE 1.
Growth rate of KT2440 and its isogenic mutants with l-lysine as the sole C and N source and l-pipecolate as a C source
| Strain | Mutant genea | Doubling time (min) in indicated culture mediumb
|
||
|---|---|---|---|---|
| M8 + l-lysine | M8 + d-lysine | M9+ l-pipecolate | ||
| KT2440 | None | 90 ± 10 | 85 ± 5 | 65 ± 5 |
| AmaC | amaC | 110 ± 5 | 120 ± 5 | 65 ± 5 |
| DpkA | dpkA | 420 ± 10 | No growth | 65 ± 5 |
| Lys2 | amaD | 325 ± 5 | No growth | 65 ± 5 |
| EEZ-PP3592 | ORF3592 | 90 ± 15 | 87 ± 35 | 66 ± 7 |
a
Inactivated gene in the isogenic mutant.
b
Doubling times were determined in exponentially growing cells. M8 medium was identical to M9 medium except that NH4Cl was omitted. Values are ± standard deviation.