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GAS MalE binds maltotriose with submicromolar affinity in an enthalpy-driven reaction. (A) Negative binding enthalpy demonstrated by representative ITC curves for GAS MalE binding with maltotriose at 12 and 24°C. (B) Plot of −ln(K) versus 1/T, showing a linear relationship. The van't Hoff enthalpy was calculated from the slope of the linear relationship to be −8.0 × 103 ± 1.0 × 103 cal/mol.
