FIG. 1.

PFGE and Southern blot hybridization analysis of the three ceftizoxime-susceptible derivatives (UP03, B5EC1, and C4EC2). (a) SacII-restricted PFGE and Southern blot hybridization of S. haemolyticus JCSC1435 and three ceftizoxime-sensitive derivatives. Southern blot hybridization was carried out using a SacII-restricted PFGE gel with PCR products obtained with SH0026, SH2171, SH2291/2292, and SH0347 as probes. Lanes M, DNA size standard (lambda ladder); lanes J, JCSC1435; lanes U, UP03; lanes B, B5EC1; lanes C, C4EC2. (b) SacII restriction sites and locations of the open reading frames used as probes in the chromosomes of JCSC1435 and ceftizoxime-susceptible derivatives. The outer circle indicates the SacII restriction sites and the locations of the open reading frames used as probes (the clockwise and counterclockwise replication arms of JCSC1435 are yellow and green, respectively). The middle circle indicates the locations of ISSha1, and the inner circle indicates the nucleotide positions (in 100-kbp increments), starting from oriC and moving clockwise.