TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Bacterial strains | ||
| Escherichia coli | ||
| DH5α | 33 | |
| TOP10 | Commercial strain used for cloning | Invitrogen |
| JM103 | 42 | |
| P. syringae pv. phaseolicola | ||
| NPS3121 | Wild type, Tox+ | 28 |
| YNorf1P | Tcr; phtA::tet polar mutant of NPS3121 | This study |
| YNorf2P | Tcr; phtB::tet polar mutant of NPS3121 | This study |
| SAorf5P | Tcr; phtE::tet polar mutant of NPS3121 | This study |
| SAorf6P | Tcr; phtF::tet polar mutant of NPS3121 | This study |
| SAorf8P | Tcr; phtH::tet polar mutant of NPS3121 | This study |
| SAorfBP | Kmr; phtK::uidA-aph polar mutant of NPS3121 | This study |
| SAorf10P | Kmr; phtL::uidA-aph polar mutant of NPS3121 | This study |
| AT3 | Kmr; amtA::aph polar mutant of NPS3121 | 12 |
| SAorf17P | Kmr; phtT::aph polar mutant of NPS3121 | This study |
| UIKsir2 | Tcr; sir2::tet polar mutant of NPS3121 | This study |
| SADesNP | Kmr; desI::uidA-aph nonpolar mutant of NPS3121 | This study |
| SAorfBNP | Kmr; phtK::uidA-aph nonpolar mutant of NPS3121 | This study |
| SAorf10NP | Kmr; phtL::uidA-aph nonpolar mutant of NPS3121 | This study |
| SAorf11NP | Kmr; phtM::uidA-aph nonpolar mutant of NPS3121 | This study |
| UIKorf1 | Kmr; phtO::uidA-aph nonpolar mutant of NPS3121 | This study |
| UIKorf4 | Kmr; phtQ::uidA-aph nonpolar mutant of NPS3121 | This study |
| SAorf17NP | Kmr; phtT::aph nonpolar mutant of NPS3121 | This study |
| Plasmids | ||
| pUC19 | Apr; 2.69 kb; lacZ′ | 42 |
| pCR4Blunt-TOPO | Apr Kmr; 3.95 kb | Invitrogen |
| pRG960sd | Smr Spr; 17.0 kb; contains a promoterless SD-uidA gene | 40 |
| pWM6 | Kanamycin cassette | 20 |
| pKL66 | Smr Spr; InReKAa; PCR fragment in pRG960sd | This study |
| pYUDF1 | Smr Spr; InReKAb; PCR fragment in pRG960sd | This study |
| pYUDF2 | Smr Spr; InReAB; PCR fragment in pRG960sd | This study |
| pSELF1 | Smr Spr; InReCD; PCR fragment in pRG960sd | This study |
| pSELF2A | Smr Spr; InRedesI-phtJ; BamHI-SspI chromosomal fragment | This study |
| pSELF4 | Smr Spr; InReTU; PCR fragment in pRG960sd | This study |