FIG. 1.

The B. anthracis Spo0E-like family. A. Amino acid sequence alignment of the B. anthracis Spo0E-like proteins. Proteins were aligned with the ClustalW program. Asterisks indicate identical residues in all five sequences; colons denote conserved substitutions. The four B. anthracis (Ba) proteins analyzed in this study are aligned against the B. subtilis Spo0E protein (35). The proteins resulting from the frameshift mutation in the BAYisI gene are indicated with the numbers 1 and 2. B. Schematic representation of the chromosomal regions containing the B. anthracis spo0E-like genes. Open reading frames and their orientations are shown by arrows. The regions forming stem-loop structures are indicated by the Ω-like symbol. The lines represent the fragments cloned in the pJM115 and pTCVlac vectors generating lacZ transcriptional fusion plasmids. The dotted bar represents the 51 codons possibly extending the BA0046 gene at the 5′ end.